Journal:
Article Title: Global genomic and proteomic analysis identifies biological pathways related to high-risk neuroblastoma
doi: 10.1021/pr900701v
Figure Lengend Snippet: The network was generated by a shortest paths algorithm of MetaCore (GeneGo) software using the list of differentially expressed proteins identified ICAT and Affymetrix microarray analysis. Nodes represent proteins; lines between nodes indicate the interaction between proteins; the arrowheads indicate the direction of the interaction. Different shapes of the nodes represent the functional class of the proteins. The nodes with circular symbol represent the differential expressed proteins obtained from our experiment; otherwise obtained from MetaCore database. The lines between the nodes indicate the interactions of the nodes; the arrowheads indicate the direction of the interaction. The color of lines between nodes describes activation (green), inhibition (red), and unspecified (gray) interactions. : over-expressed; : under-expressed; : transcription factor.
Article Snippet: DNA microarray analysis Biotinylated cRNA was synthesized and hybridized to Affymetrix Genechip human genome U133 plus 2.0 (Affymerix, Santa Clara, CA) according to the Affymetrix protocol.
Techniques: Generated, Software, Microarray, Functional Assay, Activation Assay, Inhibition